Skip to main content
. 2014 Aug 28;3(3):502–515. doi: 10.1016/j.stemcr.2014.07.008

Figure 4.

Figure 4

HDAC2 Regulates Sox10 Expression, and Sox10 Knockdown in NSCs and Oli-neu Cells Prevents Effects of T3 and VPA on Oligodendrocyte Differentiation

(A) qRT-PCR analysis of Sox10 mRNA levels in NSCs treated with T3 and/or VPA.

(B) qRT-PCR analysis of Sox10 mRNA levels in NSCs transfected with control siRNA (ECFP) or siRNAs to Hdac2 and/or Hdac3 in the presence or absence of T3.

(C) qRT-PCR analysis of Hes5 mRNA levels in NSCs treated with T3 and/or VPA.

(D) Quantification of NSCs expressing MBP following transfection with control siRNA (ECFP) or Sox10 siRNAs in the presence of T3 and VPA relative to nuclear DAPI staining.

(E) qRT-PCR demonstrating the effects of Sox10 siRNA on expression of Mbp, Plp, Sox8, Cnpase, and Sox9 following VPA and/or T3 treatment.

All experiments: n = 3–5 independent experiments. Statistical analysis: one-way ANOVA analysis of variance with Bonferroni's multiple comparison test; ∗p < 0.05. Error bars, SEM.