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. 2014 Aug 21;3(3):404–413. doi: 10.1016/j.stemcr.2014.07.006

Figure 1.

Figure 1

Increased Reprogramming with Transient p53 Suppression in Defined Conditions

(A) Timeline showing the reprogramming in defined conditions with the episomal plasmids hOCT4, hSOX2, hKLF4, hL-MYC, and hLIN28 (hOSKUL) with or without (w/wo) a short hairpin to p53 (shp53).

(B–D and F–H) Morphology of normal human dermal fibroblasts (NHDFs) and induced pluripotent stem cells (iPSCs) at days 7, 17, and 28 after reprogramming w/wo shp53.

(E and I) Tra-1-81 staining of primary iPSC colonies 28 days after reprogramming w/wo shp53.

(J) Growth rate of cells electroporated with hOSKUL, hOSKUL + shp53, or a fibroblast line stably expressing shp53 (PLK.O) at days 7, 21, and 28 after reprogramming.

(K) Alkaline phosphatase staining and counting of primary iPSC colonies at day 28 after reprogramming w/wo shp53.

Scale bars, 200 μm (B, C, F, and G) and 400 μm (D, E, H, and I). Y error bars depict SD of three independent experiments. ∗p < 0.05. See also Figure S1.