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. 2014 Aug 7;3(3):432–443. doi: 10.1016/j.stemcr.2014.07.002

Figure 2.

Figure 2

Characterization of i90c17 Human Induced Pluripotent Stem Cells and Neural Stem Cells and Mesenchymal Stem Cells Derived from i90c17 hiPSC Line

(A) Phase-contrast image of an i90c17 hiPSCs colony on feeder cell.

(B) Immunostaining showing the expression of the pluripotency markers TRA1-60, NANOG, SSEA4, and OCT4 in i90c17 hiPSC line.

(C) G-banding chromosome analysis of i90c17 hiPSCs showing a normal karyotype (46, XX).

(D) In vitro embryoid body formation from i90c17 hiPSCs showed three germ layer differentiation as illustrated by the presence of endodermal AFP+ cells, neuroectodermal PAX6+ cells, and mesodermal SMA+ cells.

(E) Phase-contrast image of hiPSCs i90c17-derived NSCs.

(F) Immunostaining showing the expression of the neural (Nestin and Sox2) and proliferating (Ki67) markers in hiPSCs i90c17-derived NSCs.

(G) Phase-contrast image of i90c17 hiPSCs-derived MSCs.

(H) Flow cytometry analysis of CD29, CD44, CD73, CD106, and CD166 expression in i90c17 hiPSCs-derived MSCs.

hiPSC, human pluripotent stem cell; MSC, mesenchymal stem cell; NSC, neural stem cell. The scale bars represent 200 μm. See also Figure S1.