Figure 4.

Methylation Quantification at the Four DMRs of GNAS in PSCs Compared to Progenies

Methylation at the GNAS locus is affected both as a function of cell type (NSC ▪ versus MSC □) and DMRs: changes in percent of methylation were observed at the NESP, XL, and A/B DMRs when comparing NSC, but not MSC, to appropriate PSCs (hiPSCs: ●; hESCs: ○); methylation at the AS DMR increases in both MSC and NSC and was significantly higher than that in PSCs (^∗∗∗p < 0.001). Cell cultures, quantification of methylation, and statistical analysis were performed as described in the Experimental Procedures section. See also Figure S3.