Figure 5.

Allelic Transcript Expression in Parental Fibroblasts, hiPSCs, and Progenies

NESP55 transcript, detected in three hiPSC clones, was monoallelic. XLsα expression was monoallelic in the two parental fibroblast (Fb) lines, biallelic in the four hiPSC clones, and returned to monoallelic expression in progenies except one (NSC, ▪). A/B expression was monoallelic in the two parental fibroblast lines, in 2/4 hiPSC clones, and in progenies (Freson et al., 2008) except one (NSC, ▪). Gsα was biallelic in the two parental fibroblast lines, the four hiPSC clones, and progenies. Cell cultures and allelic transcript expression were performed as described in the Experimental Procedures section. Expression for GNAS transcripts are expressed as percent maternal allele ratio, based on the allele expression of the maternally expressed NESP55 transcript (see Experimental Procedures for details). Biallelic expression was defined for maternal allele ratio comprised between 40% and 60% and monoallelic expression for maternal allele ratio comprised between 90% and 100% and 0% and 10%. See also Figures S4 and S5.