Table 1.
Somatic, Pluripotent Stem, and Differentiated Cells Studied
| Somatic Cells | PSCs | Differentiated Cells (Progenies) | Allelic Expression | Overall Transcript Levels |
|---|---|---|---|---|
| h fibroblasts IMR90 (XX) | hiPSC i90c01 (retrovirus) | MSC/NSC | Fb/hiPSC/MSC | Fb/hiPSC/MSC/NCS |
| hiPSC i90c17 (episome) | MSC/NSC | Fb/hiPSC/MSC/NSC | Fb/hiPSC/MSC/NSC | |
| h fibroblasts GM04603 (XY) | hiPSC 4603c27 (retrovirus) | MSC/NSC | Fb/hiPSC/MSC/NSC | Fb/hiPSC/MSC/NSC |
| hiPSC 4603 polyF (retrovirus) | Fb/hiPSC | Fb/hiPSC | ||
| hESC VUB01 (XY) | NSC | 0 | Fb/hESC/NSC | |
| hESC W09 (XX) | MSC | 0 | Fb/hESC/MSC | |
| hESC RC9 (XY) | 0 | |||
| hESC SA01 (XY) | NSC | 0 | Fb/hESC/NSC |
For each cell line, whether overall (shown in Figure S1) and allelic (shown in Figure 5) transcript expressions were analyzed is indicated. Methylation at the GNAS DMRs was studied for all cells except hiPSC i90_c01 NSC. Fb, fibroblast; h, human; hESC, human embryonic stem cells; hiPSC, human induced pluripotent stem cells; MSC, mesenchymal stem cells; NSC, neuronal stem cells. “0” indicates homozygosity at the DNA level (all hESC). Only heterozygous cells at the DNA level were analyzed for transcript expression. Overall and allelic expressions were analyzed as described in the Experimental Procedures section.