Table 1.
Antibodies used in this study.
| Antibody | Immunogen | Provenance | Dilution* | Host |
|---|---|---|---|---|
| DAGLα | C-terminus 42 amino acids of mouse DAGLα (1003–1044 amino acid residues); affinity purified with antigen polypeptide | DGLa-Rb-Af380, Frontier Institute, Ishikari, Hokkaido, Japan | I: 1/200W: 1/200 | Rabbit |
| MAGL | N-terminus 35 amino acids of mouse MAGL (1–35 amino acid residues); affinity purified with antigen polypeptide | MGL-Rb-Af200, Frontier Institute | I: 1/200W: 1/200 | Rabbit |
| β-actin | Modified β-cytoplasmic actin N-terminal peptide (DDDIAALVIDNGSGK, conjugated to KLH) | A5316, Sigma-Aldrich, St. Louis, MO | W: 1/2,000 | Mouse |
| Mouse cone-arrestin (LUMIj) | C-terminus of the mCAR protein, residues 369–381; affinity purified with the immunogen | Dr. Cheryl M. Craft, Mary D. Allen Laboratory for Vision Research, Doheny Eye Institute, USC, Los Angeles, CA | I: 1/1000 | Rabbit |
| Recoverin | Full-length recombinant human recoverin | AB5585, Millipore, Billerica, MA | I: 1/2,000 | Rabbit |
| Calbindin | Recombinant rat calbindin D-28k full length | CB-38a, Swant, Bellinzona, Switzerland | I: 1/1,000 | Rabbit |
| PKCα | Synthetic peptide with the sequence DFEGFSYVNPQFVHPILQSSV from the human protein | Sc-8393, Santa Cruz Biotechnology, Santa Cruz, CA | I: 1/500 | Mouse |
| Syntaxin | Synaptosomal plasma fraction of rat hippocampus | S0664, Sigma-Aldrich | I: 1/500 | Mouse |
| Brn-3a | Sequence: GGSAHPHPHMHGLGHLSHPAAAAAMNMPSGLPHPGLVAA fuzed to the T7 gene 10 protein | MAB1585, Millipore | I: 1/100 | Mouse |
| Glutamine synthetase (GS) | Full protein purified from sheep brain | MAB302, Millipore | I: 1/3,000 | Mouse |
| CtBP2 | Amino acids 431–445 of rat CtBP2 coupled to keyhole limpet hemocyanin via added N-terminal Cys-residue; affinity purified with the immunogen | 193003, Synaptic Systems, Göttingen, Germany | I: 1/10,000 | Rabbit |
| PSD95 | Recombinant rat PSD-95 | MAB1596, Millipore | I: 1/200 | Mouse |
| VGlut1 | Synthetic peptide representing amino acids 456–560 of rat vesicular glutamate transporters (VGlut1); affinity purified with the immunogen | 135303, Synaptic Systems | I: 1/10,000 | Rabbit |
| MAP2 | Bovine microtubule-associated protein 2 | M1406, Sigma-Aldrich | I: 1/500 | Mouse |
| PCNA | Synthetic peptide with the sequence LVFEAPNQEK | M0879, Dako, Burlington, ON, Canada | I: 1/500 | Mouse |
| Alexa Fluor 488 donkey anti-mouse | Mouse (used against PKCα, syntaxin, Brn-3a, GS, PSD95, MAP2 and PCNA) | A-21202, Molecular Probes, Eugene, OR | I: 1/500 | Donkey |
| Alexa Fluor 488 donkey anti-rabbit | Rabbit (used against mouse cone-arrestin, recoverin, calbindin, CtBP2 and VGlut1) | A-21206, Molecular Probes | I: 1/500 | Donkey |
| Alexa Fluor 647 donkey anti-rabbit | Rabbit (used against DAGLα and MAGL) | A-31573, Molecular Probes | I: 1/500 | Donkey |
| Alexa Fluor 647 donkey anti-goat | Goat (used against DAGLα and MAGL, for same hosts labeling) | A-21447, Molecular Probes | I: 1/500 | Donkey |
| Peanut agglutinin (PNA) | No immunogen; binds to galactosyl (b-1,3) N-acetylgalactosamine, rhodamine labeled | RL-1072, Vector Laboratories, Burlingame, CA | I: 1/5,000 | - |
*I, immunohistochemistry; W, Western blot.