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. 2014 Dec 15;5:693. doi: 10.3389/fmicb.2014.00693

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Copyright © 2014 Brileya, Camilleri, Zane, Wall and Fields.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Formation of coculture biofilm over time. (A–C) Electron micrographs of fixed coculture at (A) early (386X, 0 h; B) intermediate (243X, 48 h; C) steady-state (336X, 240 h) time points. (D–F) Fluorescence micrographs of coculture biofilm (D,E) embedded in polyacrylamide and hybridized with domain-specific oligonucleotide probes labeled green for D. vulgaris and red for M. maripaludis at (D) early (0 h) and (E) intermediate (48 h) time points. (F) Intact hydrated biofilm unfixed and stained with Acridine Orange at steady-state (240 h).