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Coculture biofilm (A) stained with CTC while intact and hydrated and showing all biomass stained with DAPI in blue, and CTC in red, or purple where both DAPI and CTC are present (B) scraped from the slide after CTC staining. (C) Zoomed in from the inset in (B) showing individual grains of red fluorescent CT-formazan in each cell. (D) Coculture biofilm scraped from the substratum, fixed and hybridized with domain-specific probes for D. vulgaris (green) and M. maripaludis (red).
