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. 2014 Dec 7;7:89. doi: 10.1186/s13045-014-0089-x

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© Visconte et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of ring sideroblasts by Prussian blue staining in Sf3b1 ^+/− compared to Sf3b1 ^+/+ mice. Bone marrow cells were extracted from femurs of Sf3b1 ^+/− (n = 5) and Sf3b1 ^+/+ (n = 5) and cells (2-3x10⁵) spotted on cytospin slides prior staining with Prussian blue. Ring sideroblasts (RS) were detected in Sf3b1 ^+/− compared to Sf3b1 ^+/+ mice. Images were taken from 2 mice per group. RS were also detected in additional mice as shown in Additional file 5: Figure S5.