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. 2014 Dec 9;6:191–203. doi: 10.2147/BCTT.S74663

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© 2014 Haxho et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Cell viability of MDA-MB-231 cells treated with OP at indicated doses in combination with 1 μM of either cisplatin, 5-FU, paclitaxel, gemcitabine, or tamoxifen using the WST-1 assay.

Notes: Cells were incubated in 96-well plates (5,000 cells/well) and allowed to adhere for 24 hours in 1× DMEM media containing 10% FCS. The media were replaced with fresh DMEM media containing 5% FCS with or without various concentrations of OP or chemodrugs for 24, 48, and 72 hours as predetermined optimally. Cell viability was expressed as a percent of control ± SEM of three independent experiments. Statistical analysis was carried out using GraphPad Prism, and results were compared by unpaired t-test.

Abbreviations: 5-FU, 5-fluorouracil; DMEM, Dulbecco’s Modified Eagle’s Medium; FCS, fetal calf serum; OP, oseltamivir phosphate; SEM, standard error of the mean.