Figure 6.

(A) H&E staining of necropsy lung for number of metastatic clusters per lung (B) in paraffin-embedded tissues taken from xenograft A2780 tumor-bearing RAGxCγ double mutant mice.

Notes: Mice were implanted with 0.5×10⁶ MDA-MB-231 TNBC cells cutaneously on the rear back flank, and OP treatment began daily (ip) 10 days postimplantation, when tumors reached 10–20 mm³. Paraffin-embedded tissue sections (5 μm) on glass slides were processed for H&E staining for each mouse necropsied at indicated necropsy day postimplantation. Stained tissue sections were photographed using AxioCam MRc5 digital color camera attached to a Zeiss Imager M2 fluorescence microscope at 400× magnification. Images are representative of at least five fields of view from two tissue sections. (B) Metastatic lung clusters as representative in the insert were microscopically counted per tissues sections (5 μm) and plotted in the graph. Statistical analysis was carried out using GraphPad Prism and results were compared by unpaired t-test. (C) Mean ± SEM of live necropsy lung weight per mouse body weight for each of the cohorts.

Abbreviations: H&E, hematoxylin and eosin; ip, intraperitoneally; OP, oseltamivir phosphate; SEM, standard error of the mean; TNBC, triple-negative breast cancer.