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Representative immunoblot depicting the effect of BMP4 (100 ng/mL) and Noggin (150 ng/mL), alone or in combination, on Smad1/5/8 phosphorylation (p-Smad1/5/8) in wells of 5 × 104 OSCs after the indicated durations of treatment. Note that total Smad1 protein and β-actin protein levels remain comparable across treatment groups.
