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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Fertil Steril. 2013 Aug 28;100(5):1468–1475. doi: 10.1016/j.fertnstert.2013.07.1978

Figure 4.

Figure 4

(A) Conventional RT-PCR analysis of Stra8, Msx1 and Msx2 mRNA levels in OSCs (2.5 × 104 cells per well) treated for 24 hours without or with 100 ng/mL BMP4, alone or in combination with 150 ng/mL Noggin (no RT, PCR analysis of RNA sample without reverse transcription used as a negative control to exclude genomic DNA contamination; β-actin, internal sample loading control). (B) Representative examples of immunofluorescence-based detection of Stra8 protein (green, arrows) in cultured OSCs, counterstained with rhodamine phalloidin (red) and DAPI (blue) to visualize cytoplasmic F-actin and nuclear DNA, respectively (scale bars, 10 μm). (C–E) Real-time (quantitative) RT-PCR analysis of changes in Stra8 (C), Msx1 (D) and Msx2 (E) mRNA levels normalized to β-actin mRNA levels in OSCs (2.5 × 104 cells per well) treated for 24 hours without or with 100 ng/mL BMP4, alone or in combination with 150 ng/mL Noggin (mean ± SEM, n = 4 independent cultures; different letters, P < 0.05).