Skip to main content
. 2014 Apr 29;118(49):14017–14026. doi: 10.1021/jp502624n

Figure 2.

Figure 2

Identification of cell surface receptors using cellular binding competition assays measured with flow cytometry. (A) Cellular binding of 93 nm anionic, carboxylate-modified NPs in MEM with increasing concentrations of BSA. (B) Cellular binding of 87 nm cationic, amine-modified NPs in MEM supplemented with 10 mg mL–1 BSA with increasing concentrations of fucoidan. (C) Cellular binding of 87 nm cationic, amine-modified NPs in MEM supplemented with 10 mg mL–1 BSA with increasing concentrations of polyinosinic acid. (D) Control experiments show cellular binding of 87 nm amine-modified NPs in the presence of polyadenylic acid (PolyA) and the autofluorescence from cells in the absence of NPs (cells only, error bar is too small to see).