FIG 3.

Investigation of CSFV-infected pDC-derived cytokines on the MHC class II expression of NK and γδ T cells. (A) pDCs or mDCs were exposed to CSFV C-strain, Brescia strain, or a mock virus-infected cryolysate supernatant, and after 24 h in culture, the levels of TNF-α and type I IFN in the supernatants were analyzed using a porcine cytokine multiplex ELISA and Mx/CAT reporter gene assay, respectively. (B) Enriched NK and γδ T cells were then cultured in cRPMI medium alone or in the presence of porcine recombinant IFN-α and/or TNF-α, at concentrations comparable to those detected in infected pDC cocultures, and the expression of MHC-II was assessed by flow cytometry after 24 h. The mean ± SEM data from three independent experiments utilizing different animals are shown. The values for each group were compared to the corresponding unstimulated control (mock stimulation in panel A and no cytokine treatment in panel B) using a one-way ANOVA, followed by Dunnett's multiple comparison test: ***, P < 0.001; **, P < 0.01; *, P < 0.05.