FIG 6.

Quantification of local and systemic type I IFN following C-strain vaccination and/or virulent CSFV Brescia challenge. Serum and tonsils were collected longitudinally following C-strain vaccination and challenge 5 days later with CSFV Brescia strain. Cells expressing IFN-α were enumerated in the interfollicular areas (closed symbols) and follicles (open symbols) of the tonsils by immunohistochemistry (A), and the levels of type I IFN in serum were determined using an Mx/CAT reporter gene assay (B). The data represent the mean of 3 pigs/group ± SEM. Statistical analyses were performed using a one-way ANOVA, followed by Bonferroni's multiple comparison test: ***, P < 0.001; *, P < 0.05.