FIG 5.

Long-lasting p11C tetramer and SIV Gag pooled peptide-stimulated IFN-γ ELISpot responses. At week 107 postprime (i.e., week 64 postboost), prior to SIV challenge, mutant rBCG- or rDNA-primed groups demonstrated sustained but mostly low levels of p11C tetramer-positive CD8⁺ T lymphocytes (A) and robust persistent IFN-γ ELISpot responses upon SIV pooled peptide stimulation with variations between groups (B), whereas such responses were lacking in the BCG Wt and BCG-pSL10 cohorts. For BCG Wt, n = 4; for the other groups, n = 5. Kruskal-Wallis test, P = 0.003; Dunn's multiple-comparison test (significant differences), P = 0.04 (BCG Wt versus J13-pSL7) or P = 0.03 (BCG-pSL10 versus J13-pSL7). Bars indicate medians.