FIG 2.

Multiparameter flow cytometry analyses of CD4 (A) and CD8 (B) splenic T cells. Spleen cells from four or five mice immunized with BCG by either the s.c. or the i.n. route were harvested 3 months postvaccination and then stimulated with BCG for about 40 h in vitro. The frequency (percentage) of monofunctional T cells producing only IFN-γ (IFNg) or cells producing both IFN-γ and TNF-α, IFN-γ (IFN/TNF) and IL-2, or TNF-α and IL-2 or all three cytokines (TP) was determined. Significant differences from naive controls (*, P < 0.05) and mice immunized with BCG s.c. (#, P < 0.05) are indicated.