Table 6. Cytotoxicity and Cell Based Potency Data for Selected Compounds.
| cytotoxicity in SHSY5Y cells after 48 h |
|||||
|---|---|---|---|---|---|
| cmpd | % cell viability at 10 μM | % cell viability at 30 μM | in-cell Western,a SHSY5Y IC50 (nM) | inhibition of 6-OHDA induced cell death, SHSY5Y IC50 (nM) | inhibition of 6-OHDA induced mitochondrial membrane depolarization,SHSY5Y IC50 (nM) |
| 22f | 7 | 5 | 866 | 2976 | 40 |
| 22g | 24 | 5 | 2331 | ndb | ndb |
| 22i | 80 | 56 | 905 | 13 | 17 |
| 26f | 84 | 16 | 3250 | ndb | 130 |
| 26j | 91 | 98 | 1436 | 568 | 25 |
| 26g | 105 | 97 | N/Ic | N/Ic | N/Ic |
| 26k | 112 | 97 | N/Ic | N/Ic | 31 |
| 26n | 92 | 98 | 1895 | 281 | 4 |
a
IC50 values are the mean of two or more experiments (with triplicate replicates for each experiment) with errors within 80% of the mean.
b
Not determined.
c
No inhibition up to 10 μM.