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. 2014 Dec 7;20:2571–2577. doi: 10.12659/MSM.892994

Figure 2.

Figure 2

(A) Impact of SIN3A gene expression changes on the proliferation ability of U251 cells. (B) Impact of SIN3A gene expression changes the apoptosis rate of U251 cells. (C) Western blot analysis for SIN3A and cleaved Caspase3 expression of U251 cells treated with anti-miR-210 and siRNA-SIN3A transfection. GAPDH was used as a reference control. (D) quantitative analysis of the relative protein levels of SIN3A and cleaved Caspase3 normalized to those of GAPDH was shown. *, ** P <0.05 vs. control U251 cells; #, ## P <0.05 vs. U251 cells with anti-miR-210. IDV is the abbreviation for “integrated density values”, which is calculated by computerized image analysis system (Fluor Chen 2.0) and normalized with that of GAPDH.