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. Author manuscript; available in PMC: 2014 Dec 15.
Published in final edited form as: Anticancer Drugs. 2010 Mar;21(3):243–251. doi: 10.1097/cad.0b013e328333d5ce

Fig.2.

Fig.2

Frequency of micronuclei in mouse polychromatic erythrocytes after rmhTNF-α treatments (0/00). Activity of rmhTNF-α to induce bone marrow micronucleus was assessed in ICR mice. Three groups (6 mice/group) received 3.5×108, 0.7×108 to 0.14×108 IU/kg i.m. administrations of rmhTNF-α respectively. Saline and cyclophosphamide (CPA, 30 mg/kg) were used as negative and positive controls. 48 hrs later, the animals were sacrificed and the thighbone marrow cells were analyzed following methanol fixing and Giemsa staining. The frequency of micronuclei was counted based on an examination of 1000 polychromatic erythrocytes (PCE) for every mouse. The results did not show any significant difference between rmhTNF-α and saline cohorts (P>0.05) which indicated no micronuclei inducing toxicity for rmhTNF-α in mice.