Fig.4.
Serum concentration time-course of three intramuscular doses (10, 20, and 40 μg/kg) or one intravenous dose (10 μg/kg) of [125I]-rmhTNF-α administration in mice. Serum from replicate animals (n=4) in each group were collected at time points of 0.25, 0.5, 1, 2, 3, 4, 6, 8, 16 hrs post-dosing. The radioactivity of serum samples (after HPLC purification) were quantified in duplicate by liquid scintillation spectrophotometer. The concentration of prototype rmhTNF-α in each samples were determined according to the radioactivity-concentration standard curve. pharmacokinetic profiles characterized by rapid tissue distribution and the maximum serum concentration of rmhTNF-α could be reached in 0.5 hr in case of intramuscular doses.