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. 2014 Dec 5;7:2215–2225. doi: 10.2147/OTT.S69530

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© 2014 Gomà et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Lipid raft isolation from the three prostate cell lines.

Notes: (A) Lipid raft enriched fractions were isolated from the normal cell line PNT2 and showed MRP1 was present only in the last lipid raft enriched fraction, enriched with Cav-1. (B) When lipid raft fractions were isolated from the androgen-sensitive prostate cancer cell line LNCaP, MRP1 was already detected in early lipid raft enriched fractions together with Cav-1 (F1–F2) and in greater amounts in a later lipid raft enriched fraction (F3). Both PNT2 and LNCaP cells presented soluble fractions of MRP1 (F5–F11). (C) Lipid raft enriched fractions isolated from the androgen-independent prostate cancer cell line PC3 showed the presence of MRP1 mainly in the first two fractions (F1–F2). Flotillin-1 (Flot-1) was used as a control of the lipid raft isolation.

Abbreviations: PNT2, normal human prostatic epithelial cell line; MRP1, multidrug resistance protein 1; Cav-1, Caveolin-1; LNCaP, lymph node carcinoma of the prostate; PC3, prostate cancer cell 3 line.