Fig. 2.

Photomicrographs of human macrophage monolayers at day 5 of exposure to β-glucan preparations. MDMs were grown in RPMI containing autologous serum in a 24-well tissue culture plate for 12 days when the cells were exposed to different concentrations of PGG or WGP. The morphology of the MDM monolayer was observed daily over 5-day period under an inverted phase microscope. Following incubation for 5 days with β-glucans, only WGP at 100 mg/ml concentration proved partially or more completely toxic for the MDMs. All other concentrations of WGP had no significant effect on the monolayer. Photomicrographs were taken at 100× magnification and are representative of two experiments.