Fig. 8.

WGP pre-treatment does not further increase ROI production with opsonized zymosan, but does further increase ROI production upon PMA stimulation. MDMs were adhered to 96-well plates (~6 × 10⁴ MDMs/well) in 10% autologous serum. MDM monolayers were repleted with RHH medium containing WGP (25 μg/ml) and incubated at 37 °C/5% CO₂ for 30 min. WGP was washed away with DPBS, and the monolayer repleted with DPBS + 32 mM DCF, and incubated for 30 min at 37 °C, 5% CO₂. MDMs were then stimulated with opsonized zymosan particles (OpZ; MOI 12:1) or PMA (200 nM or 0.123 μg/ml), or no treatment (control). Relative fluorescence, indicative of ROI production, was measured every 2 min for 90 min. A) RFU profile obtained from MDMs stimulated with either opsonized zymosan or WGP alone as well as from WGP pre-treated MDMs stimulated with opsonized zymosan. B). RFU profile obtained from MDMs stimulated with either PMA or WGP alone as well as from WGP pre-treated MDMs stimulated with PMA. Graphs show the mean values of RFUs from triplicate wells from a representative experiment (n = 2).