Table 1. Characteristics of selected C19MC microRNAs.

Assay name	miRBase ID	NCBI Location Chromosome	microRNA sequence	Expression in placenta
hsa-miR-516-5p	hsa-miR-516b-5p	Chr.19: 58920508 - 58920592 [+]	5′-CAUCUGGAGGUAAGAAGCACUUU-3′	exclusively expressed
hsa-miR-517*	hsa-miR-517-5p	Chr.19: 54215522 - 54215608 [+]	5′-CCUCUAGAUGGAAGCACUGUCU-3′	high expression
hsa-miR-518b	hsa-miR-518b	Chr.19: 54205991 - 54206073 [+]	5′-CAAAGCGCUCCCCUUUAGAGGU-3′	exclusively expressed
hsa-miR-520a*	hsa-miR-520a-5p	Chr.19: 54194135 - 54194219 [+]	5′-CUCCAGAGGGAAGUACUUUCU-3′	high expression
hsa-miR-520h	hsa-miR-520h	Chr.19: 54245766 - 54245853 [+]	5′-ACAAAGUGCUUCCCUUUAGAGU-3′	exclusively expressed
hsa-miR-525	hsa-miR-525-5p	Chr.19: 54200787 - 54200871 [+]	5′-CUCCAGAGGGAUGCACUUUCU-3′	exclusively expressed
hsa-miR-526a	hsa-miR-526a	Chr.19: 54209506 - 54209590 [+]	5′-CUCUAGAGGGAAGCACUUUCU-3′	high expression

C19MC microRNAs were divided into two categories (microRNAs exclusively expressed in the placental tissue and those with high expression in the placental tissue) based on information in miRNAMap 2.0 database (http://mirnamap.mbc.nctu.edu.tw/index.php), where the Q-PCR experiments for monitoring the expression profiles of 224 human miRNAs in eighteen major normal tissues in humans are provided. For example, we indicated miR-516-5p and miR-518b as those to be exclusively expressed in the placental tissue, since according to the miRNAMap 2.0 database miR-516-5p was shown to be expressed only in the placental tissue and miR-518b to be highly expressed in the placental tissue and rarely expressed in testes. On the other hand, for instance miR-520a* showed besides high expression in the placental tissue also low expression in other human tissues involving adipose, bladder, brain, cervix, heart, kidney, liver, lung, muscle, ovary, prostate, small intestine, spleen, testes, thymus, thyroid and trachea.