a–c, Focusing to a single absorber. The exterior fields at instant t1 (a) and t2 (b) are measured to calculate ΔE. ΔE* is produced to promote TRAP focusing (c). d, e, Principle of DOPC. The light paths follow the solid arrows. In the probing mode (d), the exterior fields are measured interferometrically to obtain the TRAP phase map. In the focusing mode (e), the SLM displays the synthesized phase map, and the reading beam is turned into a phase-conjugated beam to form a focus. See Methods for details. f, Light intensity distribution measured on the focal plane when a single focus is formed. g, h, Focal intensity profiles sampled along y and x directions in f. Dashed lines are Gaussian fits. i, Focal intensity distribution showing focusing at two points due to a moving target. L, lens; BS, beamsplitter. Scale bars, 50 μm.