Table 2.

Effects of fish oil on bone gene expression after 9 months of supplementation in 6T and B6.

	B6		6T		P Genotype	P Oil	P Int
Gene	Safflower	Fish	Safflower	Fish
Igf-1	0.98±0.07	1.43±0.11**	1.50±0.10	1.74±0.13	0.0004	0.002	NS
Il-6	1.05±0.10	1.03±0.13	1.14±0.11	1.27±0.11	NS	NS	NS
Opg	1.18±0.22	0.61±0.13*	1.36±0.29	0.71±0.16*	NS	0.01	NS
Pparγ	1.08±0.14	0.62±0.03**	1.66±0.08	1.31±0.07**	0.0001	0.0001	NS
Rankl	1.05±0.10	0.84±0.10	1.05±0.06	0.94±0.11	NS	NS	NS
AdipoQ	1.03±0.08	1.19±0.08	1.52±0.15	2.03±0.18*	0.0001	0.01	NS
Adipsin	1.15±0.15	1.15±0.11	5.78±1.4	7.07±0.94	0.0001	NS	NS
Il-10	1.16±0.17	0.24±0.03***	0.43±0.04	0.31±0.04*	0.002	0.0001	0.0003
Il1-ra	1.04±0.14	3.11±0.43****	1.78±0.43	4.33±0.66**	0.05	0.0001	NS
Tnfα	1.00±0.02	0.82±0.04***	1.04±0.05	1.33±0.08**	0.0001	NS	0.0001
Mcsf	1.01±0.04	0.74±0.06**	1.00±0.04	1.11±0.07	0.001	NS	0.001
Trap	1.04±0.09	0.74±0.03**	1.17±0.06	1.23±0.02	0.0001	0.03	0.002
Ak2	1.11±0.13	1.16±0.10	1.34±0.24	1.30±0.17	NS	NS	NS
Fabp4	1.03±0.08	1.87±0.05***	1.43±0.18	2.19±0.19**	0.01	0.0001	NS
Fsp27	1.03±0.07	1.05±0.08	3.34±0.70	5.31±0.69*	0.0001	0.05	0.05
Lpl	1.08±0.13	1.49±0.15*	1.25±0.16	1.55±0.16	NS	0.02	NS
Pepck	1.15±0.24	1.64±0.20	2.54±0.61	11.64±1.61****	0.0001	0.0001	0.0001

Gene expression was evaluated by qRTPCR (n=6–8 per group).

^*p<0.05 significant difference vs safflower in each genotype;

^$p<0.05 significant difference vs B6 in each diet by post-hoc fisher’s PLSD (2F-ANOVA, genotype and diet). Interaction, Int. Means ± SEM.