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Of 19,378 PB1 sequences analyzed, 19,339 contain a start codon at position 95, signaling the beginning of the PB1-F2 ORF. The downstream stop codons vary, resulting in different ORF sizes. The 3 major PB1-F2 lengths are 90, 57 and 11 aa. It is generally assumed that an intact PB1-F2 is ≥79 aa in length.
