Table 1. Animal characteristics under chow-fed conditions.
| FXR+/+ | FXR−/− | |||
| Morphometric parameters | WT | PBS | FXRα2 | FXR4 |
| Body weight (g) | 28.0 (26.0–28.9) | 27.2 (25.0–30.5) | 27.1 (24.0–28.7) | 26.5 (25.4–27.6) |
| Liver weight (% BW) | 4.9 (4.5–5.5) | 5.5 (4.9–6.0) | 5.1 (4.3–5.5) | 5.0 (4.8–5.3) |
| Plasma | ||||
| triglycerides (mmol/l) | 0.6 (0.5–1.0) | 1.4 (1.0–2.6)* | 1.1 (0.9–1.2)* # | 0.8 (0.7–1.6)* # |
| cholesterol (mmol/l) | 2.6 (1.9–3.1) | 7.6 (7.2–7.9)* | 3.7 (3.4–3.9)* # | 5.8 (5.4–6.5)* #$ |
| Liver | ||||
| triglycerides (µmol/g) | 10.3 (6.6–12.6) | 13.6 (7.6–15.9) | 7.4 (6.5–9.0)* # | 8.7 (7.6–12.2) |
| cholesterol (µmol/g) | 7.1 (6.5–8.1) | 5.1 (4.7–5.4)* | 6.1 (5.7–6.9)* # | 6.3 (5.9–7.9)* # |
| Biliary output | ||||
| bile flow (ml/day/100 g) | 14.8 (12.9–19.5) | 14.6 (10.7–16.6)* | 19.2 (17.2–24.0)* # | 14.6 (12.4–17.8)* #$ |
| bile salts (µmol/day/100 g) | 435.1 (262.4–518.3) | 482.6 (291.8–778.4) | 309.3 (258.9–612.9) | 405.7 (338.1–475.4) |
| cholesterol (µmol/day/100 g) | 8.5 (6.4–13.2) | 8.1 (5.7–8.8) | 10.8 (8.4–11.7) | 9.1 (7.0–11.8) |
| phospholipids (µmol/day/100 g) | 76.6 (69.3–91.6) | 79.9 (72.9–91.1) | 100.6 (82.6–122.6)* # | 98.3 (84.3–148.3)* # |
Animal characteristics of chow-fed FXR knock-out mice injected with PBS or stably transduced with hepatic-specific scAAV-FXR2 or scAAV-FXR4 were compared to chow-fed wild-type (WT) mice. Values are presented as median (range) (n = 5–6 animals per group).
*p<0.05 vs. WT,
#
p<0.05 vs PBS,
$
p<0.05 between isoforms.