Table 2. Hepatic and intestinal expression profile of genes involved in bile salt metabolism.

	chow-fed			0.5% cholic acid-fed
Liver	PBS	FXRα2	FXRα4	PBS	FXRα2	FXRα4
Nr1h4/Fxr**	1.0±0.2	36.1±7.1*	50.0±14.9*	1.0±0.5	31.9±7.7*	29.8±10.8*
Nr0b2/Shp	1.0±0.3	1.8±0.8	1.4±1.0	1.0±0.7	5.0±0.8*	4.1±1.0*
Abcb11/Bsep	1.0±0.2	2.9±0.2*#	2.1±0.5*	1.0±0.3	6.7±0.9*#	3.8±0.8*
Cyp7a1	1.0±0.4	0.7±0.3	0.6±0.3	1.0±0.7	0.02±0.02	0.2±0.1
Cyp7b1	1.0±0.4	0.9±0.2	1.1±0.2	1.0±0.4	0.9±0.2	0.3±0.0*#
Cyp8b1	1.0±0.2	0.4±0.1*#	0.6±0.3*	1.0±0.4	0.01±0.01*#	0.03±0.01*
Intestine	PBS	FXRα2	FXRα4	PBS	FXRα2	FXRα4
Nr1h4/Fxr**	1.0±0.2	0.7±0.1	0.8±0.1	1.0±1.0	23.5±7.7	20.9±17.5
Fgf15	1.0±0.7	0.4±0.3*	0.4±0.3*	1.0±0.9	2.9±1.1*	1.5±1.0
Fabp6/Ibabp	1.0±0.3	3.0±0.9*	2.3±0.9*	1.0±0.9	2.4±1.0*	1.2±0.5*

Hepatic and intestinal gene expression determined by quantitative real-time PCR of genes involved in synthesis, transport and signaling of bile salts in chow- and 0.5% cholic acid-fed PBS-injected and stably transduced scAAV-FXRα2 or scAAV-FXRα4 FXR knock-out mice. Gene expression levels were normalized to 36b4 and corrected to expression levels of PBS-injected FXR knock-outs. Values are presented as average ± standard deviations (n = 5–6 animals per group). *p<0.05 vs. PBS; ^#p<0.05 between FXR isoforms. ** note that our QPCR primers pairs always picked up FXR signal, even in FXR knock-outs. Expression levels were normalized to expression levels found in PBS-injected FXR knock-out mice.