Figure 5.

The collagen type I in HSC conditioned medium induced EMT. A. Morphological change of ML1 cells after 24 h of 100-300 μg/ml collagen type I treatment; B. Immunobloting of E-cadherin, N-cadherin, Slug and b-actin in ML1 cells. C. siRNA was used to knockdown fibronectin or collagen type I expression in HSC-T6 cells, and culture supernatant was collected to treat ML1 cells for 24 h. The morphological changes of ML1 cells are shown in 200x magnification, and immunobloting of E-cadherin, N-cadherin, Slug and collagen type I. The knockdown efficiency was confirmed by western blotting of collagen type I expression in conditioned medium.