Validation of the extent in altered gene expression from arrays by quantitative real-time RT-PCR. Total RNA was extracted from thyroids of wild-type, KrasG12D, ThrbPV/PV, and ThrbPV/PV
KrasG12D mice. Eight representative genes: (A) Integrin α6, (B) Integrin β4, (C) Integrin αV, (D) Integrin β1, (E) Integrin β3, (F) Integrin α5, (G) Fibronectin, and (H) Myc.