Figure 5.

Protein abundance of integrin-ERBB2 signaling pathway in wild-type, Kras^G12D, Thrb^PV/PV, and Thrb^PV/PV Kras^G12D mice thyroids. Protein levels were measured using antibodies against (A) ERBB2, (B) Fibronectin, (C) Integrin α6, (D) Integrin β4, (E) Integrin αV, (F) Integrin β1, (G) Integrin β3, (H) Integrin α5, and (I) loading control GAPDH. Total protein extracts were prepared from thyroids of wild-type and Kras^G12D mice and thyroid tumors of Thrb^PV/PV and Thrb^PV/PV Kras^G12D mice, and the Western blot analysis was carried out as described in Materials and Methods.