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. 2014 Nov 19;4(6):886–896.

Figure 4.

Figure 4

The influence of AR CAG repeat length in androgen-induced TMPRSS2 and ERG gene co-localization. A. TMPRSS2 (green) and ERG (red) co-localization rates in DU145 cells with and without the transfection of AR with 15 (15 CAG), 17 (17 CAG) and 24 (24 CAG) CAG repeats were assessed by FISH with and without DHT treatment. -DHT: cells cultured without androgen using charcoal striped serum; +DHT: cells cultured with charcoal striped serum and additional DHT at 100 nM; ****: P < 0.0001; B. Representative FISH images to show cells without TMPRSS2 and ERG co-localization (17 CAG repeats AR transfected cells without DHT treatment) and with a pair of TMPRSS2 and ERG co-localized (17 CAG repeats AR transfected cells with DHT treatment).