Table 1.
mTOR pathway: sensibility in detecting biomarkers by three tissue-based techniques
1 Molecules | Immunohistochemistry (IHC) | Western blot (WB) | Immunofluorescence (IF) |
---|---|---|---|
mTOR | - | - | ++ |
p-mTOR | ++ | - | // |
p70S6k | - | ++ | // |
p-p70S6k monoclonal | ++ | - | + |
p-p70S6k polyclonal | - | - | // |
p-S6RP | - | ++ | // |
p-4EBP1 | - | ++ | // |
//: not recommend.