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Expression profile of LiTAT. In all cases a 1:3000 dilution and 1:10000 of specific anti-LiTAT and anti-gGAPDH polyclonal antibody respectively was used. 10 μg of total protein extracts were loaded per well. (A) L. infantum early-logarithmic and stationary phase promastigotes and axenic amastigotes protein extracts. (B) L. infantum stationary PNA+ and PNA− protein extracts. (C) L. chagasi early-logarithmic stage of resistant and sensitive strains.
