Table 3.
Allelic diversity (expected heterozygosity, He) and allelic richness (Rs) at 8 neutral microsatellite loci in various chromosomes within the genome in samples collected at two different time points.
| Sample population – 1995 (n = 47) |
Sample population – 2013 (n = 94) |
|||
|---|---|---|---|---|
| Microsatellite locus | Allelic richness (Rs) | Expected heterozygosity (He) | Allelic richness (Rs) | Expected heterozygosity (He) |
| Population sampled (n = number of individual isolates) | ||||
| Poly-α | 12.0 | 0.819 | 13.0 | 0.839 |
| PfPK2 | 9.0 | 0.739 | 12.0 | 0.861 |
| ARA2 | 11.0 | 0.791 | 11.0 | 0.688 |
| TA87 | 9.0 | 0.702 | 18.0 | 0.847 |
| TA42 | 14.0 | 0.760 | 12.0 | 0.649 |
| 2490 | 11.0 | 0.715 | 13.0 | 0.661 |
| TA60 | 11.0 | 0.785 | 9.0 | 0.510 |
| TA109 | 14.0 | 0.849 | 18.0 | 0.780 |
| Mean ± SD | 11.4 ± 1.9 | 0.77 ± 0.05 | 13.3 ± 3.2 | 0.73 ± 0.12 |
The sample population represents the evaluable genotypes in the two time points. Though the original total samples available for genotyping was 96 and 119 in 1995 and 2012/2013 respectively, samples presenting >1 allele at any of the 8 loci were excluded leading to the loss of a substantial number of samples (ultimately n = 47 and n = 94 in 1995 and 2012/2013, respectively). This sampling variance, however, did not occasion any significant difference between the mean He in 1995 and 2013.