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. 2014 Dec 12;34(6):e00158. doi: 10.1042/BSR20140152

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Determination of the on-rate constants (k_on) for mant-GMPPNP with (A) wild-type and (B–G) mutant EcNFeoB proteins. Figure shows observed association rate constants (k_obs) for mant-GMPPNP with EcNFeoB proteins versus concentration. (Insert) Traces of mant-GMPPNP binding at the indicated final protein concentrations. Observed rate constants (k_obs) were determined from the single exponential taken from each trace and plotted as a function of protein concentration. The observed rate constants have a linear relationship from which k_on can be determined.