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. 2014 Dec 12;34(6):e00158. doi: 10.1042/BSR20140152

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Dissociation of mant-GDP from (A) wild type and (B–G) mutant EcNFeoB proteins. The off-rate constant (k_off) was determined directly from the single exponential function of the data (see Table 1). Final k_off values are the average of three or more stopped-flow experiments with each experiment consisting of five or more replicates.