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. 2014 Dec 12;34(6):e00159. doi: 10.1042/BSR20140130

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Western blot detected the expression of FN, Col I, CTGF and CyclinD1 of each experimental group at 24 h after 10 ng/ml TGFβ1 treatment. The expressions of those proteins of EP2-siRNA+TGFβ1 group were increased significantly than that of NC-siRNA+TGFβl group. (A) Western Blot. (B–E) Quantification of FN, Col I, CTGF and CyclinD1 expression is achieved using densitometric values normalized to β-actin levels. (F–I) The relative mRNA levels of FN, Col I, CTGF and CyclinD1 are also shown (aP<0.05 versus control; ^bP<0.05 versus TGFβ1 group; ^cP<0.05 versus NC-siRNA+TGFβ1group).