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. 2014 Dec 12;34(6):e00159. doi: 10.1042/BSR20140130

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Western blot detected the expression of FN, Col I, CTGF and CyclinD1 of each experimental group at 24 h after 10 ng/ml TGFβ1 treatment. The expressions of those proteins of AD-EP2+TGFβ1 group were decreased significantly than that of AD-GFP+TGFβ1 group. (A) Western blot. (B–E) Quantification of FN, Col I, CTGF and CyclinD1 expression is achieved using densitometric values normalized to β-actin levels. (F–I): The relative mRNA levels of FN, Col I, CTGF and CyclinD1 are also shown (aP<0.05 versus AD-GFP group; ^bP<0.05 versus AD-EP2 group; ^cP<0.05 versus AD-GFP+TGFβ1group).