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. 2014 Dec 12;34(6):e00159. doi: 10.1042/BSR20140130

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© 2014 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Primary MCs were transfected with EP2-siRNA for 12 h and then incubated with 10 ng/ml TGFβ1 for 24 h. The expression of COX2 and mPGES1 in EP2-siRNA+TGFβ1 group were significantly increased than that of NC-siRNA+TGFβ1group. (A) Western blot (B) Quantification of COX2 and mPGES1 expression is achieved using densitometric values normalized to β-actin levels. (C) The relative mRNA levels of COX2 and mPGES1 are also shown (aP<0.05 versus control; ^bP<0.05 versus TGFβ1 group; ^cP<0.05 versus NC-siRNA+TGFβ1group).