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. 2014 Dec 4;13:170. doi: 10.1186/s12934-014-0170-8

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© Mi et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Production of geraniol and geranic acid by P. putida DSM 12264 expressing GES and MVA. Indicated are the concentrations of de novo produced geraniol (blue) and geranic acid (red) over time using P. putida DSM 12264 expressing GES and MVA. Cells were grown in shake flasks in E2 medium (containing 10% LB, 30 mg/L kanamycin and 9.2 g/L glycerol) at 30°C and heterologous gene expression was induced 3 h after inoculation with 0.2% (w/v) L-rhamnose. Geraniol and geranic acid concentrations were determined via HPLC over time. Mean values of two independent experiments, indicated by error bars, are given. For detailed statistical analysis, please see Additional file 4.