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. Author manuscript; available in PMC: 2015 Nov 20.
Published in final edited form as: Cell. 2014 Nov 13;159(5):1110–1125. doi: 10.1016/j.cell.2014.10.013

Figure 5. Signal-induced BCAR4-SNIP1 Interaction Attenuates the Inhibitory Effect of SNIP1 on p300 HAT Activity.

Figure 5

(A and B) RIP-qPCR detection of the indicated RNAs retrieved by Myc-specific antibody in MDA-MB-231 cells electroporated with indicated vectors followed by CCL21 treatment.

(C) In vitro HAT activity assays of p300 in the presence of wt SNIP1, full-length (FL) BCAR4 and their corresponding mutants as indicated.

(D) In vitro HAT activity assays of p300 in the presence of wt SNIP1 and its corresponding mutants as indicated.

(E) IB detection of the interaction between SNIP1 (a.a. 97-274) and p300 (a.a. 1198-1806) wt or truncations.

(F) In vitro HAT activity assays of p300 in the presence of SNIP1 a.a. 97-274 and BCAR4 sense or antisense RNAs.

(G) ChIP-qPCR detection of acetylated histone marks occupancy on the promoters of selected GLI2 target genes in MDA-MB-231 cells treated with CCL21.

(H) ChIP-qPCR detection of phospho-GLI2, p300 and H3K18Ac occupancy on PTCH1 promoter in MDA-MB-231 cells transfected with indicated siRNAs followed by CCL21 treatment.

Error bars, SEM of three independent experiments (*p<0.05 and **p<0.01).

See also Figure S5.