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. 2014 Dec 1;12(4):238–250. doi: 10.1089/lrb.2014.0012

FIG. 3.

FIG. 3.

Age-related changes in lymphatic vessels. 9-week-old mice were allowed to age. Lung tissues were collected at indicated time points, and lymphatic vessels were identified with anti-LYVE-1 antibodies. Images of the entire lung section were obtained. ImageJ software was used to measure the density (A, D, and G), perimeter (B, E, and H), and area (C, F, and I) of total (A–C), subpleural (D–F), and interstitial (G–I) lymphatic vessels in lung tissue sections at the indicated time points (n=3 per group per time point). Results showed that between 10 weeks and 25 weeks of age, there was no significant change in total, subpleural, or interstitial lymphatic vessel density (A, D, and G). Throughout the study period, there was no significant change in subpleural lymphatic vessel perimeter (E) or area (F). A statistically significant age-related increase in the perimeter (B and H) and area ratio (C and I) of total and interstitial lymphatic vessels was observed; *p<0.05; **p<0.01, by 1-way ANOVA. (These data are different representations of the same data from control animals in Figs. 1 and 2. These graphs are meant to illustrate the maturation effects on lung lymphatic vessels).