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HIV-1 Tat-induced IL-10 secretion by monocytes is TLR4-dependent. Monocytes pre-incubated or not with blocking antibodies against TLR4 (1 μg/ml) or isotype-matched mAb were treated with HIV-1 Tat protein (10 nM), GST-Tat 1–45 (10 nM) or GST (10 nM) as control. After 24 h, IL-10 in the supernatant was measured by ELISA. The data represent means ± SD of three independent experiments.
