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. 2014 Dec 16;8:404. doi: 10.3389/fnins.2014.00404

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Copyright © 2014 Saunders, Dreifuss, Dziegielewska, Johansson, Habgood, Møllgård and Bauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Behnsen's much cited trypan blue experiments. (A) Brain from postnatal mouse injected systemically 3 times with trypan blue at P4–P14. (B) Adult mouse brain following systemic injection of trypan blue. Note that as described by Behnsen the dye staining in the postnatal brain is in the same regions as in the adult brain, but more extensive. This is probably a visual artifact due to the use of an outline of an adult mouse brain for both ages. In postnatal mouse brain (P14) the cerebral cortex and cerebellum are less developed than illustrated here. Note also that in the postnatal brain there does not appear to be any dye staining of the neocortex-the region of the developing brain with the least mature blood vessels. From Behnsen (1927).