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. 2014 Dec 15;211(13):2507–2517. doi: 10.1084/jem.20140137

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© 2014 Moalli et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — TP^−/− OT-II CD4⁺ T cells display increased activation marker expression as compared with WT OT-II CD4⁺ T cells. (A-C) WT and TP^−/− OT-II CD4⁺ T cells were analyzed by flow cytometry 24 h after adoptive transfer in presence of DCs pulsed with low (0.1 µM) and high (10 µM) OVA_323-339 concentrations. A shows representative flow cytometry plots of CD25 and CD69 expression. Numbers indicate percent positive cells. Percentages and normalized MFI of CD69⁺ (B) and CD25⁺ (C) transferred WT and TP^−/− OT-II CD4⁺ T cells are shown. Each dot in B and C represents PLNs pooled from one mouse. Data are from 2–3 independent experiments with a total of 6–8 mice and analyzed using repeated measure ANOVA with Bonferroni post-test. Bars indicate mean. *, P < 0.05; **, P < 0.01; ***, P < 0.001.